Coding
Part:BBa_K1125004:Design
Designed by: Yi-Yuan Lee Group: iGEM13_NTU_Taiwan (2013-09-16)
5.8S rDNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 384
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 490
Design Notes
First, we get this sequence from PCR. Then we use site-directed mutation to exclude the invalid site. Third we composed this sequence into pGAPZA.
Source
From Rodotorula glutinis genomic DNA.