Coding

Part:BBa_K1125004:Design

Designed by: Yi-Yuan Lee   Group: iGEM13_NTU_Taiwan   (2013-09-16)


5.8S rDNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 490


Design Notes

First, we get this sequence from PCR. Then we use site-directed mutation to exclude the invalid site. Third we composed this sequence into pGAPZA.


Source

From Rodotorula glutinis genomic DNA.

References